The purpose of this study was to empirically examine the process model of meaning-making in work, and the impact on individual and organization in Japanese firms. 267 employees from 25 to 39 years old, completed the web-based survey on meaning-making process (positive interpretation of work, self-worth in workplace, awareness of one’s purpose in work, self-worth in organization), and the impact on individual (meaning in life) and organization(desirable organizational behavior) through the meaning outcome (in-depth meaning of working). The major results were, 1) the meaning-making in work proceeds from self-worth in the workplace, positive interpretation of work, awareness of one’s purpose in work, and to self-worth in organizations, 2) awareness of one’s purpose in work and self-worth in organizations enhanced in-depth meaning of working, and self-worth in organizational behavior promoted desirable organizational behavior directly or indirectly, and 3) self-worth in organization and organizational behavior influenced meaning in life.